In this blog, Anna Zueva, researcher at the A.N. Severtsov Institute of Ecology and Evolution (Moscow), reveals the hidden world of viruses in eusocial insects. Read her latest article in Insectes Sociauxhere.
Eusocial insects are a kind of human society in miniature. Each part of the community has its own functions and features. The system of social insects’ family works as a coherent and well-coordinated mechanism, and the life of each individual strongly depends on the life of the whole complex.
The functioning of even a fine-tuned system can be disrupted. For example, in the history of mankind there are known episodes when whole civilizations suffered from local epidemics and even pandemics caused by microscopic entities – viruses. But is that the case for social insects?
Our work started from our interest in viruses of invertebrates. Tropics are especially promising for us, as this region is known for its great biodiversity and has lots of still unknown biological species, including of course microorganisms and viruses.
We started our investigation with the research on viruses of termites of Cát Tiên National Park – a part of the Đồng Nai Biosphere Reserve (Fig. 1). We are grateful to the South branch of the Joint Russian-Vietnamese Tropical Research and Technological Center for invaluable help in our studies.
We took samples of termites of three different species feeding on different substrates – lichens and fungi – as we expected that food resource can affect the composition of viruses associated with insects (Fig. 2, 3).
Thought we did not observed any visible symptoms of infection, we detected four new viruses related to viruses previously discovered in termites. We also found the evidence of presence of virus probably belonging to termites’ food substrate (Litov et al. 2022), which partly support our suggestion about the effect of feeding type on the insect virome.
Potentially, eusocial insects can be a model for studying the spread of viruses via social interactions. In the recently published review (Zueva et al 2024), we aimed to actualize the information on the diversity of viruses associated with termites and ants, which are among the most functionally important soil invertebrates. In our review we analyzed 93 articles dedicated to viral findings in both groups of insects. To date, viruses were detected in 54 ant species and in 28 species of termites. We have pointed out 270 viruses and viral genetic variants detected in soil-dwelling social insects, and less than one third of them were associated with termites (Fig. 4). It is obvious that the virome of termites is still mostly undescribed. In addition, both for ants and termites, the information on symptoms or on replication of viruses in their insect hosts remains strongly limited. More studies of the virome of soil-dwelling eusocial organisms with more attention to viral replication and infection symptoms are needed (Zueva et al. 2024).
The most amazing is, that despite the presence of numerous potentially harmful viruses and intense interactions between individuals within a colony, evidences of massive viral epidemics in termites are virtually unknown. We found the information only about one possible virus-caused termite family extinction (Chouvenc et al. 2013). This is especially surprising since termites are important pests of human structures and agriculture, and the search for viruses that infect them has been ongoing for a long time.
Figure 4. Visualization of the presence of viruses in ants and termites (based on Zueva et al. 2024).
There are numerous other unsolved questions on the virome of social insects. How many more viruses of eusocial insects we still don’t know about? Are they able to cause acute dangerous infections or are they just present in the insect tissues and do not manifest themselves until the immune system of host is critically compromised?
We are planning to continue our work in tropics, both on social insects and beyond them. We are sure that this region is a great source of new virological investigations, both on social and solitary invertebrates. By the way, a recent research of our team revealed at least eight new viruses in millipedes collected in the Cat Tien National Park (Litov et al. 2024).
References:
Chouvenc T, Mullins AJ, Efstathion CA, Su NY (2013) Virus-like symptoms in a termite (Isoptera: Kalotermitidae) field colony. Florida Entomologist 96(4):1612–1614. https://doi.org/10.1653/024.096.0450
Litov A.G., Semenyuk I.I., Belova O.A., Polienko A.E., Thinh N.V., Karganova G.G., Tiunov A.V. (2024) Extensive diversity of viruses in millipedes collected in the Dong Nai Biosphere Reserve (Vietnam). Viruses, 16: 1486. https://doi.org/10.3390/v16091486.
Litov AG, Zueva AI, Tiunov AV, Van Thinh N, Belyaeva NV, Karganova GG (2022) Virome of Three Termite Species from Southern Vietnam. Viruses 14(5):860. https://doi.org/10.3390/v14050860
In this blog, Mariane Dias-Soares and Cléa Mariano explore the diverse organisms cohabiting ant nests in the Neotropics, from gastropods to myriapods. They explain how do these guests interact with ants, sharing resources and space within the nest environment. Discover more about these intriguing interactions in their latest work for Insectes Sociaux, here.
What attracts these other groups? What are these groups? Are there really gastropods inside ant nests? What are commensals? Do ants benefit from their presence? Why aren’t they expelled? These are some of the most frequent questions when the topic of conversation is our research and our article. Let’s now address each of these questions, the work done so far, and the next steps toward the discoveries that researching an ant nest provides us…
The ant nests provide a protected environment for the workers, the queen, and all of their immatures, as well as storing food and maintaining stable temperature and humidity. When studying these nests, the presence of other groups was observed, which, attracted by these resources, coexist with the ants. These groups may spend part of their life cycle inside the nests or even their entire existence.
Gastropod near the immatures of N. verenae. Photo: Laís Bomfim
Our research aims to identify which groups are associated with different ant species in a Neotropical region. In my master’s studies, I focused on the species Neoponera verenae, an ant from the subfamily Ponerinae that nests in various substrates such as dry cocoa pods, soil, and decomposing logs. In our study, we found a variety of groups, including Myriapoda, Isopoda, Araneae, Lepidoptera, Pseudoscorpiones, Collembola, Acari, Coleoptera, Diptera, Dermaptera, and Gastropoda, among others. This highlights the great diversity of organisms that coexist within these ant nests.
Caterpillar in a N. verenae nest near workers and immatures. Photo: Mariane Dias-Soares]Researchers during new field collections in the Neotropical Region of Brazil. Photo: Mariane Dias-Soares
Noticing the high number of groups within the ant nests sparked in us the need not only to identify which groups inhabit them but also to understand the interactions that occur in these environments. In our article, we studied the facultative commensalism of gastropods in N. verenae nests, presenting novel records and proposing hypotheses about this type of interaction.
There are different types of interactions between ants and gastropods. In the case of facultative commensalism, the gastropods coexist peacefully with the ants, benefiting from the protection provided by the colony, the available food, and the environmental stability, while also being found outside the nests. For the ants, however, we did not observe any apparent benefit or loss. Further research will delve deeper into these issues.
Gastropods recorded inside N. verenae ant nests. (yellow arrows indicate immatures, and orange arrows indicate snails). Photos from the article by Dias-Soares et al. (2024)
Through various observations and records made in the field and laboratory, we found the presence of several gastropod species inside the ant nests. Among the gastropods found, the family Achatinidae was the most abundant. These gastropods coexisted harmoniously with the workers and the young individuals in the nest (larvae, pupae, and eggs), moving freely without being disturbed by the ants. We also observed that the gastropods produced a foam, which generated a pacifying effect that prevented their expulsion from the nests. This is one of the strategies used by these organisms to inhabit ant nests.
Our study presents novel records of the interaction between ants and gastropods, leading us to explore various unresolved questions. One of these questions is the degree of interaction between immature ants and gastropods, as we found individuals in the chambers that contained the immatures. Additionally, we are investigating the chemical nature of the mucus involved in these interactions and identifying the new species of gastropods found in the nests, in collaboration with Dr. Sthefane D’ávila. Ongoing studies focus on analyzing the chemical strategies used, the morphological adaptations and behaviors exhibited, and the existence of mimicry within these nests. There is still much to be discovered in the vast world that is an ant nest…
Some members of the research team currently conducting collections for the new phase of the Project. from left to right: Fred da Silva, Mariane Dias-Soares and Jossiane DiasPart of the research group led by Cléa Mariano and Jacques Delabie, focusing on studies of various ant species and other groups present in ant nests
Tommy is a researcher at the University of Regensburg, where he leads the ACElab since 2016. He study value perception and decision-making in invertebrates (mostly ants). His latest work in Insectes Sociaux can be found here.
IS: Who are you, and what do you do?
My name is Tomer (Tommy, please) Czaczkes, and I study the behaviour of mostly ants, sometimes bees, and very occasionally other arthropods. My current focus is on comparative psychology – understanding how animals think, learn, and make decisions. I’m trying to apply our hard-earned knowledge of behavioural ecology to controlling invasive ants. I also dabble in collective behaviour.
Tommy Czaczkes thinking about Lasius fuliginosus.
IS: How did you develop an interest in your research?
Ah, well. During my undergraduate the average grades for different modules were available, and I noted that while cell biology and microbiology had pretty low average grades, behaviour and ecology had quite high ones. I know which side of the bread is buttered, and, honestly, I never expected to stay in research. Then, during my undergraduate project, I realized that while the miserable vertebrate ecology people would have to trek for hours through the forest to sight their animal, I, as an experimental behavioural ecologist working on ants, could collect 50 datapoints in half a day, while drinking rum.
IS: What is your favorite social insect, and why?
Oooh, a tough question! I’m torn between two ant species: Lasius niger and Pheidole oxyops.L. niger is perhaps the most common ant in Europe, and as my PhD supervisor Francis Ratnieks always says “it’s the common animals that are most interesting. They’re clearly doing something right.”. L. niger are extremely smart, polite, helpful, and make excellent colleagues. P. oxyops, however, do wonderful cooperative transport – the collective carrying of loads. They have an amazing, explosive recruitment behaviour, and love cheese. They’re also extremely common, but alas, in Brazil and not in Germany, where I’m based.
Pheidole oxyops carrying a 10x10mm square of choose by the corners (published in Insectes Sociaux).
IS: What is the best moment/discovery in your research so far? What made it so memorable?
Seeing ants being visibly disappointed when they received food which was poorer than what they were expecting – poor things! It was clear from the moment I did the first pilot on that project that we would have a clear and strong effect. It was memorable because it was simply so easy to relate to: the disappointed ants would check the food, break away, try again to make sure, and circle around looking for the good stuff they were sure was there before. It was simply so cute and relatable.
IS: Do you teach or do outreach/science communication? How do you incorporate your research into these areas?
I enjoy going into schools and kindergartens, to talk to kids about ants and insects in general. It’s always fun to bring an ant colony or two, and show the “mama ant” and her babies. For the bigger kids, it’s fun to do a pheromone following assay – makes me feel like an ant whisperer, who can use my super science powers to talk to insects.
IS: What do you think are some of the important current questions in social insect research, and what is essential for future research?
This is showing my own biases here, but I think the question of insect sentience and intelligence is a huge question, and social insects are central to the experimental examination of these topics. We’ve had a slew of high impact work reporting all sorts of impressive cognitive abilities, with a big swing from behaviourism to cognition. I expect that very soon the swing will move the other way again, with people starting to push for simpler explanations, or attempting replication studies. Animal behaviour as a subject is overdue a big replication study, the likes of which shook up the worlds of experimental psychology and cancer research (amongst others) recently. I have attempted to replicate some of my own work, with some things replicating wonderfully, and others simply not there next time I looked. And yes, I publish the failed replications too.
Lasius niger worker who is very satisfied with her drop of sucrose solution.
IS: Outside of science, what are your favorite activities, hobbies, or sports?
I really enjoy hiking in the mountains, when I can get out. When not, I’m a big fan of sci-fi books and computer games. My mind is still somewhat blown by my VR set.
IS: What is the last book you read? Would you recommend it? Why or why not?
I’m almost through “Delusions of Gender” by Cordelia Fine. The book speaks against the supposed ‘evidence’ for a simplistic biological basis for gender roles. Would I recommend it? It’s convincing and helpful, but sometimes feels like being bludgeoned with an endless series of (reasonable) criticisms of studies. It’s well researched and useful, but perhaps not the page turner it could have been.
IS: How do you keep going when things get tough?
Give up! No, really. On days where I can’t focus, I simply stop working. If an experiment runs into wall after wall, I’ll drop it. But for things like rejections, failures, etc – I take the long view, and remind myself that this is normal, and this too shall pass. Oh, and moaning. Moaning helps.
IS: If you were to go live on an uninhabited island and could only bring three things, what would you bring? Why?
Assuming my basic survival needs were met? My ebook stuffed with books (for entertainment), a solar charger to charge it, and a Swiss army knife to bootstrap other tools from. I think I’ve played too much Minecraft.
IS: Who do you think has had the most considerable influence on your science career?
Certainly my Doctoral supervisor, Prof. Francis Ratnieks. He has an absolutely excellent eye for interesting biology. Moreover, I admire (and have tried to emulate) his quick, cheap, and cheerful approach to research projects – avoiding the huge, long term, ultra-high tech projects, and preferring short, fun, and simple projects which require only some ants, a few strips of paper, and some drops of sucrose. And a good idea, of course.
In this experiment, Tommy’s team was testing whether ants prefer food they have worked harder for (they do). A good example of their experimental designs. Note the Lego, paper runways, and complete lack of high tech gubbins.
IS: What advice would you give to someone hoping to be a social insect researcher in the future?
Read “The Ants” by Hölldobler and Wilson. Yes, it’s almost 35 years old, but it’s a wonderful primer to most of the major topics in social insect biology. I read it cover to cover to prepare for my PhD, and that knowledge has stood me in good stead since then.
IS: Has learning from a mistake ever led you to success?
Not nearly as much as I would have hoped. I seem doomed to making the same mistakes over and over again. However, at least by now I recognise them with absolute clarity in hindsight.
IS: What is your favorite place science has taken you?
The La Selva biological field station in Costa Rica, where I did my Bachelors project (on leaf cutter ants). Being surrounded by researchers for the first time, in a beautiful jungle, with amazing animals, was life changing. I also met my future wife there, so that was a nice bonus.
Oscar Vaes is a biologist interested in data analysis and scientific communication. He has just completed his PhD in Belgium. His latest work on “inactive” ants in colonies can be found here.
IS: Who are you, and what do you do?
I’m a Belgian biologist and recently finished my PhD about activity levels in the red ant Myrmica rubra, at the Université Libre de Bruxelles. At present, I’m trying to put my knowledge of data analysis to good use, an aspect of research that I really enjoy and in which I’m trying to improve.
IS: How did you develop an interest in your research?
Simply by working on them. Basically, I’m curious to understand how things around me work, hence my interest in biology. This, combined with my attraction to animals, meant that I was predisposed to take an interest in social insects. However, it was really when I was looking for a research topic for my master thesis that I developed an interest in ants.
IS: What is your favorite social insect, and why?
So far, I’ve only worked on one biological model, Myrmica rubra, and although it doesn’t treat me in the best way during my experimental manipulations or field harvests, I still have to choose it. Being only at the beginning of my research career, I feel I’ve only glimpsed the tip of the iceberg, so I’m sure this favorite animal will evolve over time. Yet, I think it will always be a species of ant. I believe that they occupy a special place in the collective unconscious and fascinate people. I never tire of seeing the reaction people have when we tell them we’re studying the behavior of ant colonies. It is always a fun icebreaker.
Queen, worker and larva of Myrmica rubra. A) Young worker carrying a larva. B) Queen without wings. C) Young worker (top) with light cuticular pigmentation and queen (bottom) standing over a larva. D) Larva (2nd instar).
IS: What is the best moment/discovery in your research so far? What made it so memorable?
My best moments are usually when I get to share with researchers from other laboratories, at conferences. These moments are always very enriching, and have the instant effect of taking us out of the tunnel vision we might have when working for months on our subject in an office.
In terms of discoveries, I based much of my PhD subject on the hypothesis that there was probably a large proportion of inactive individuals in colonies of the ant I worked with. Having confirmation that around 30% of our species’ colonies form a distinct group of nurses, foragers, and domestics, and that we could cross-reference their characteristics with those of other species, was one of those really exciting moments when the prospects for future experiments develop and become clearer.
IS: Do you teach or do outreach/science communication? How do you incorporate your research into these areas?
Passing on knowledge is something I really enjoy doing. I’ve always been attracted to teaching, without actually doing it professionally. As a result, I try to value the moments when I can explain my research and simplify it. I find that being able to explain complex phenomena in a simple way is a great asset, but it also reflects the fact that we ourselves have understood things in depth. So practicing simplifying/explaining research is also a way of assessing one’s own level of knowledge.
IS: What do you think are some of the important current questions in social insect research, and what is essential for future research?
There’s no particular research topic that stands out for me, and this is no doubt linked to the fact that I don’t yet have a global vision of the study of social insects. However, the development of computer tools has made it much easier to acquire certain types of data by automating their collection and processing in much greater quantities than was possible in the past. I think we need to keep a critical eye on the effect these tools have on the observer, his or her ability to interpret results or even spot phenomena. I have several examples in mind of times when I’ve spent weeks turning over data presented in spreadsheets in search of answers to questions we were asking ourselves, only to have the answer right under my nose all along on the videos of my colonies. Although computer tools are a great help most of the time, they tend to distort our vision of results. There’s nothing like the eye of the experimenter to give you a first-hand view of the phenomena you’re about to dissect!
IS: Outside of science, what are your favorite activities, hobbies, or sports?
I’d say bicycles are one of my main interests. Basically, it’s always been my means of transport in Brussels, but as I was working with it, I became interested in the mechanical side of things. This basically means I have several unfinished project bikes laying in a corner of my garage. Recently, I’ve been enjoying discovering the Belgian countryside by bike, and I have to say that it’s a fantastic tool for that. I also enjoy discovering new sports and eSports disciplines. I love the feeling of beginning to understand the reasoning behind the actions of professional athletes or players, of developing a form of expertise in a new discipline. Since it’s also more fun to share interests with others, I often get sucked into people’s passions.
IS: What is the last book you read? Would you recommend it? Why or why not?
It’s not related to my research topic, but the last book I read was written by Victoria Defraigne, and is an explanatory book on transidentity. Knowing it was written by a student at my university was the trigger to finally learn about a subject I knew was full of stereotypes and misinformation in my mind. I think that for the moment, this book only exists in French, but I urge people to get informed about a subject still full of misunderstandings.
IS: How do you keep going when things get tough?
To be honest, I think I’m lucky in that I never really have a hard time. I’m very privileged in life, with family and friends all around me, which makes it easy for me to put things into perspective when they don’t go as planned. So far, the difficult periods have all been relatively limited in time, with a clearer horizon in sight each time. So, I find it easier to accept the situation and tell myself that it’s only temporary, as all the previous tough times have been.
IS: If you were to go live on an uninhabited island and could only bring three things, what would you bring? Why?
I always have my pocket magnifier in my backpack, and I use it more often than you’d think. I’d have a hard time parting with it, so I’m going to choose this as my first item. I hope, of course, that by “uninhabited” we’re talking about humans and not local wildlife. In two, I’d say coffee beans, so as to quickly have a plantation to support myself. I wouldn’t accomplish much on a desert island without my morning coffees. As I have no idea about the third, I think I’d let friends choose for me, so I’d have a surprise on arrival, good or bad.
IS: Who do you think has had the most considerable influence on your science career?
I think my PhD promoter, Claire Detrain, takes first place hands down. Then I’d say it’s the rest of the team in her lab. When it came time for me to find a subject for my master thesis in the various laboratories at my university, I gave as much importance to the atmosphere and ambience within the team, as to the research subject. Today, I’m very happy to have followed my intuition, and as a bonus, I’ve taken an interest in our six-legged friends.
IS: What advice would you give to someone hoping to be a social insect researcher in the future?
Not to work on Myrmica rubra! On a more serious note, I don’t think there are any tips specific to the study of social insects. The only mistake I see being made frequently is that of systematically trying to draw parallels between our behavior and that of social insects, but it’s mainly made by a non-scientific audience. I imagine that anyone interested in social insects quickly realizes that a large part of their charm lies in the fact that their group is structured in such a way as to modify the implications that collective responses have on individuals and the group. Over the last few years, I’ve supervised a number of students who have all shown themselves to be very curious and eager for results when working on ants, without having any prior interest in these insects. So, I think we’re lucky to be working with animals that naturally arouse people’s interest and curiosity, which can only be a good thing.
Myrmica rubra workers with the colored tags we use during experiments.
IS: Has learning from a mistake ever led you to success?
Of course it did. One example I really like is when we first started doing individual marking of ants, and we were looking for the best way to do it. We struggled quite a bit with methods we used to perform in our lab, and finally decided to reach out to another researcher who seemed to have great results with a different technique, but whom we had never spoken to. Not only was he willing to give us a detailed explanation of his techniques, but we were able to implement many tips that completely changed our way of tagging. We have been training young researchers to tag ants with great success and will probably be using these tips for many years to come.
In a broader sense, I think that making mistakes reinforces our ability to question ourselves, something that is key when doing science. I find that conducting research helps us accept mistakes and learn from them. Moreover, I believe this translates into being more open-minded in life, deconstructing deep-rooted misconceptions, and being more apt to listen to others.
IS: What is your favorite place science has taken you?
I have a very ‘first degree’ answer to this question. I mentioned earlier my fondness for conferences, and I was lucky enough to attend IUSSI San Diego in 2022. So, I’d say it was one of the highlights of my thesis, where I was able to meet many people whose research inspired me, but also to discover the research subjects of laboratories from all over the world. I really enjoyed communicating my results to an international audience of social insect experts, whose feedback inevitably led to enriching and constructive discussions.